Experimental Approaches for Studying PI3K–AKT

Researchers use multiple experimental strategies to investigate PI3K–AKT signaling.

Western Blotting

Western blot analysis can be used to examine the abundance and phosphorylation state of signaling proteins such as AKT and downstream pathway components.

Immunofluorescence

Immunofluorescence can provide information about the cellular localization of signaling proteins and changes associated with pathway activation.

ELISA-Based Assays

Phosphorylation-specific immunoassays can provide quantitative measurements of signaling components.

Gene Expression Analysis

RT-qPCR can be used to investigate transcriptional changes associated with cellular responses to pathway modulation.

Pharmacological Modulation

Experimental inhibitors and activators can help researchers investigate pathway relationships and downstream effects.

Proteomics

Mass spectrometry-based proteomics can provide a broader view of signaling-associated changes across cellular systems.